Purpose: The purpose of this study was to explore nursing students’ experience in microbiology courses. Methods: Data were gathered through 4 focus group interviews and 1 in-depth personal interview, by 19 nursing students who attended microbiology courses. Data were collected June 15-July 20, 2018. Conventional content analysis was used for data analysis. Results: The result of this study revealed 4 categories: “facing the challenge”, “types of learning”, “lack of learning motivation”, “acquiring knowledge of infection”. Conclusion: Findings suggest that it is important to identify nursing students’ perspectives, to improve microbiology curriculum in the educational process. Also, it is necessary to connect continuously, between educational and practical environments, for effective management of microbiology courses.