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Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
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  • Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
저자명
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae
간행물명
Journal of Veterinary ScienceKCI,SCIE,SCOPUS
권/호정보
2018년|19권 1호(통권70호)|pp.59-70 (12 pages)
발행정보
대한수의학회|한국
파일정보
정기간행물|ENG|
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영문초록

Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than that of wild-type (WT) B. henselae. To study the differences in proteomic expression between WT and Δomp43, two-dimensional gel electrophoresis-based proteomic analysis was performed. Based on Clusters of Orthologus Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and 3 proteins associated with cellular processing and signaling. By semi-quantitative reverse transcriptase polymerase chain reaction, increases in tldD, efp, ntrX, pdhA, purB, and ATPA mRNA expression and decreases in Rho and yfeA mRNA expression were confirmed in Δomp43. In conclusion, this is the first report showing that a loss of OMP43 expression in B. henselae leads to retarded proliferation. Furthermore, our proteomic data provide useful information for the further investigation of mechanisms related to the growth of B. henselae.

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