Purpose : Cyclosporine A (CsA) is a widely used immunosuppresive agent with development of gingival overgrowth as
a side effect. The mechanisms underlying this immunosuppressive activity are not fully understood. The progression of
gingival overgrowth results from the accumulation of extracellular matrix. The purpose of this study was to investigate
the mRNA expression of several growth factors associated with CsA according to the induction time and dose.
Materials & Methods : Gingival fibroblasts were obtained from gingival tissues of healthy donor and the patients treated
with CsA. The cultured gingival fibroblasts were incubated with increasing concentration(0, 250, 500, 750, 1000, 1500,
2000 ng/ml) of CsA for 24, 48, and 72 hours, and the expression of MMP-1, TIMP-1, TGF-β1, FGF-2 and FGF-7 was
determined by reverse transcriptase-polymerase chain reaction (RT-PCR).
Results : The expression of MMP-1 was slightly decreased according to the concentration of treated CsA in 24 and 48
hours stimulation. There was no change in TIMP-1 expression. TGF-β1 showed the increased expression in 72 hours
stimulation and FGF-2 expression was increased in 48 and 72 hours stimulation. The FGF-7 expression was
significantly decreased in 48 and 72 hours stimulation.
Conclusions : We concluded that the gingival overgrowth might be related with TGF-β1, MMP-1 and FGF-2
expression associated with collagen metabolism. And the FGF-7 associated with keratinocyte growth was significantly
related with CsA gingival overgrowth. We also speculated that there was no cellular change associated with CsA
induction in gingival fibroblast.